Πλοήγηση ανά Συγγραφέα "Dimitriou, T. G."
-
Characterization of novel intergenogroup and intergenotype recombinant noroviruses from central Greece
Ruether, I. G. A.; Dimitriou, T. G.; Tsakogiannis, D.; Kyriakopoulou, Z.; Amoutzias, G. D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2014)Noroviruses (NoVs) are a major causative agent of acute gastroenteritis in humans. They are members of the Caliciviridae family and based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided into ... -
Circulation of intergenotype recombinant noroviruses GII.9/GII.6 from 2006 to 2011 in central Greece
Ruether, I. G. A.; Tsakogiannis, D.; Kyriakopoulou, Z.; Dimitriou, T. G.; Papamichail, C.; Gartzonika, C.; Leveidiotou-Stefanou, S.; Markoulatos, P. (2014)Noroviruses (NoVs) are members of the Caliciviridae family and are recognized as a worldwide cause of acute nonbacterial gastroenteritis. Based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided ... -
Determination of human papillomavirus 16 physical status through E1/E6 and E2/E6 ratio analysis
Tsakogiannis, D.; Kyriakopoulou, Z.; Ruether, I. G. A.; Amoutzias, G. D.; Dimitriou, T. G.; Diamantidou, V.; Kotsovassilis, C.; Markoulatos, P. (2014)Human papillomavirus (HPV) 16 genome integration into the host chromosome is a crucial event during the life cycle of the virus and a major step towards carcinogenesis. The integration of HPV16 DNA promotes a constitutive ... -
Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66
Tsakogiannis, D.; Papacharalampous, M.; Toska, E.; Kyriakopoulou, Z.; Dimitriou, T. G.; Ruether, I. G. A.; Komiotis, D.; Markoulatos, P. (2015)Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three ... -
Identification of novel E6-E7 sequence variants of human papillomavirus 16
Tsakogiannis, D.; Kyriakopoulou, Z.; Amoutzias, G.; Ruether, I. G. A.; Dimitriou, T. G.; Panotopoulou, E.; Markoulatos, P. (2013)The rate of evolution of the human papillomavirus 16 (HPV16) genome is low. However, the ability of the E6 oncoprotein to interact with distinct p53 variants causes selective pressure on the E6 gene. In addition, intratypic ... -
Molecular and evolutionary analysis of HPV16 E6 and E7 genes in Greek women
Tsakogiannis, D.; Papadopoulou, A.; Kontostathi, G.; Ruether, I. G. A.; Kyriakopoulou, Z.; Dimitriou, T. G.; Orfanoudakis, G.; Markoulatos, P. (2013)Human papillomavirus type 16 (HPV16) non-European variants have been associated with persistent infection and cervical cancer development, while the L83V variant of the E6 gene has been correlated with the progression of ... -
Molecular epidemiology and evolutionary dynamics of Echovirus 3 serotype
Kyriakopoulou, Z.; Bletsa, M.; Tsakogiannis, D.; Dimitriou, T. G.; Amoutzias, G. D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2015)Echovirus 3 (E3) serotype has been related with several neurologic diseases, although it constitutes one of the rarely isolated serotypes, with no report of epidemics in Europe. The aim of the present study was to provide ... -
Multiplex PCR assay for the rapid identification of human papillomavirus genotypes 16, 18, 45, 35, 66, 33, 51, 58, and 31 in clinical samples
Tsakogiannis, D.; Diamantidou, V.; Toska, E.; Kyriakopoulou, Z.; Dimitriou, T. G.; Ruether, I. G.; Gortsilas, P.; Markoulatos, P. (2015)The causal association between persistent human papillomavirus (HPV) infection and cervical cancer has lead to the development of a variety of molecular assays for HPV detection. The present study focused on the development ... -
Nucleotide polymorphisms of the human papillomavirus 16 E1 gene
Tsakogiannis, D.; Darmis, F.; Gortsilas, P.; Ruether, I. G. A.; Kyriakopoulou, Z.; Dimitriou, T. G.; Amoutzias, G.; Markoulatos, P. (2014)The E1 ORF is one of the most conserved regions in the human papillomavirus (HPV) genome. The complete E1 gene of the HPV16 genome was amplified with four overlapping primer sets in 16 high-grade (CIN II, III) and 13 ... -
PCR assays for the identification of rare recombination types from VP1 to 3D genomic region of vaccine derived poliovirus strains
Dimitriou, T. G.; Pliaka, V.; Kyriakopoulou, Z.; Ruether, I. G. A.; Tsakogiannis, D.; Fountoucidou, P.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2014)Poliomyelitis has been effectively controlled by the use of inactivated poliovirus vaccine (IPV) or trivalent live attenuated oral poliovirus vaccine (OPV). Since 1964, the use of OPV in mass vaccinations has resulted in ... -
Prevalence of HPV16 E1-1374 boolean AND 63nt variants in Greek women
Tsakogiannis, D.; Kyriakopoulou, Z.; Darmis, F.; Ruether, I. G. A.; Dimitriou, T. G.; Orfanoudakis, G.; Panotopoulou, E.; Markoulatos, P. (2014)Recent studies have focused on sequence variation of the HPV16 E1 gene. The present study investigates the prevalence of E1-1374<^>63nt duplication in the Greek population, and the sequence variation at the 5 ' end of the ... -
Sites of disruption within E1 and E2 genes of HPV16 and association with cervical dysplasia
Tsakogiannis, D.; Gortsilas, P.; Kyriakopoulou, Z.; Ruether, I. G. A.; Dimitriou, T. G.; Orfanoudakis, G.; Markoulatos, P. (2015)Integration of HPV16 DNA into the host chromosome usually disrupts the E1 and/or E2 genes. The present study investigated the disruption of E1, E2 genes in a total of eighty four HPV16-positive precancerous and cervical ...